Nucleic acid isolation kit with columns, 50 or 100 reactions

Nucleic acid isolation kit with columns, 50 or 100 reactions

Catalog number: AVT-E50, AVT-E100

Description

The kit is designed to isolate DNA / RNA from various types of biomaterial: from biological fluids, cell cultures or solid tissues. The kit allows efficient extraction of the smallest amount of nucleic acids through the use of affinity membrane column technology. The obtained nucleic acid can be further used for PCR, cloning, sequencing, restriction analysis. The small volume of elution allows to obtain a concentrated sample. The kit includes all the necessary plastic consumables for extraction.

Passing time 40 min
Materials required to use the kit
  • Class II biosafety cabinet.
  • Microcentrifuge with an acceleration of at least 10,000 g
  • Solid thermostat for test tubes (25-100) ° C or water bath at 70 ° C.
  • Microcentrifuge vortex.
  • Dispensers on 1-20 μl, 20-200 or μl, 200-1000 μl.
  • Sterile tips with filters for 20, 200 and 1000 μl
  • Disposable gloves without talc.
  • A separate set of PPE.
  • Tripods for tips and test tubes with a volume of 1.5 ml.
  • Disposal container.
  1. Lysis buffer
  2. Buffer for precipitation
  3. Washing buffer
  4. Eluent buffer
  5. Extraction columns
  6. Test tubes for washing